RESUMO
Burkholderia pseudomallei is a causative agent of melioidosis. The present study investigated IL-1beta mRNA and protein expression by peripheral blood mononuclear cells and purified monocytes (n = 10) in response to infection with B. pseudomallei and B. thailandensis. Similarly increased IL-1beta mRNA and protein expression was found in both PBMC and purified monocytes stimulated with B. pseudomallei and B. thailandensis. Thus, this study suggests that IL-1beta response does not differ between infections with B. pseudomallei and its non-virulent counterpart and other mechanisms may be involved in their distinct virulence in causing the disease.
Assuntos
Burkholderia pseudomallei/imunologia , Burkholderia/imunologia , Interleucina-1beta/biossíntese , Leucócitos Mononucleares/imunologia , Monócitos/imunologia , Burkholderia pseudomallei/patogenicidade , Células Cultivadas , Humanos , Leucócitos Mononucleares/metabolismo , Leucócitos Mononucleares/microbiologia , Monócitos/metabolismo , Monócitos/microbiologia , RNA Mensageiro/metabolismoRESUMO
Burkholderia pseudomallei is the causative agent of melioidosis. One of the main risk factors for B. pseudomallei infection in endemic areas is diabetes mellitus. The present study investigated IL-17 mRNA and protein expression by peripheral blood mononuclear cells in response to B. pseudomallei infection in 10 diabetic patients in comparison to 10 healthy blood donors. The IL-17 expression in diabetic patients was significantly lower (p < 0.05) than in the controls. However, IL-23 mRNA expression of the 2 groups was comparable. The present findings suggest that melioidosis affects T cell IL-17 production and that patients with diabetes mellitus have a defective IL-17 production in response to this type of infection.
Assuntos
Burkholderia pseudomallei/imunologia , Diabetes Mellitus Tipo 1/imunologia , Diabetes Mellitus Tipo 2/imunologia , Interleucina-17/sangue , Leucócitos Mononucleares/imunologia , Melioidose/imunologia , Adulto , Humanos , Interleucina-17/genética , Interleucina-23/sangue , Leucócitos Mononucleares/metabolismo , Leucócitos Mononucleares/microbiologia , Melioidose/complicações , Melioidose/metabolismo , Melioidose/microbiologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Linfócitos T/imunologiaRESUMO
PROBLEM: As there has been a study in mice showing the expression of IL-17 by decidual cells and the status of IL-17 receptor expression in human pregnancy is not known, we hypothesized that IL-17 may regulate human trophoblast proliferation and invasion. METHOD OF STUDY: JEG-3 cell line was used as a model for human trophoblast. Immunohistochemitry and reverse transcriptase polymerase chain reaction techniques were used to identify IL-17 receptor protein and mRNA, respectively. The effects of IL-17 on JEG-3 cell proliferation and invasion were tested using the BrdU incorporation and the Matrigel invasion assays, respectively. RESULTS: IL-17 increased the invasive capacity of JEG-3 cells but had no effect on the proliferation and multinucleated formation of JEG-3 cells. CONCLUSION: In this JEG-3 cell model of human trophoblast, the IL-17R and IL-17 may have a regulatory role in trophoblast invasion.
